KMID : 0545120190290010011
|
|
Journal of Microbiology and Biotechnology 2019 Volume.29 No. 1 p.11 ~ p.20
|
|
Ecklonia cava Extract Containing Dieckol Suppresses RANKL-Induced Osteoclastogenesis via MAP Kinase/NF-¥êB Pathway Inhibition and Heme Oxygenase-1 Induction
|
|
Kim Seon-Young
Kang Seok-Seong Choi Soo-Im Kim Gun-Hee Imm Jee-Young
|
|
Abstract
|
|
|
Ecklonia cava, an edible marine brown alga (Laminariaceae), is a rich source of bioactive compounds such as fucoidan and phlorotannins. Ecklonia cava extract (ECE) was prepared using 70% ethanol extraction and ECE contained 67% and 10.6% of total phlorotannins and dieckol, respectively. ECE treatment significantly inhibited receptor activator of nuclear factor-¥êB ligand (RANKL)-induced osteoclast differentiation of RAW 264.7 cells and pit formation in bone resorption assay (p <0.05). Moreover, it suppressed RANKL-induced NF-¥êB and mitogen-activated protein kinase signaling in a dose dependent manner. Downregulated osteoclast-specific gene (tartrate-resistant acid phosphatase, cathepsin K, and matrix metalloproteinase-9) expression and osteoclast proliferative transcriptional factors (nuclear factor of activated T cells-1 and c-fos) confirmed ECE-mediated suppression of osteoclastogenesis. ECE treatment (100 ¥ìg/ml) increased heme oxygenase-1 expression by 2.5-fold and decreased intercellular reactive oxygen species production during osteoclastogenesis. The effective inhibition of RANKL-stimulated osteoclast differentiation and oxidative stress by ECE suggest that ECE has therapeutic potential in alleviating osteoclast-associated disorders.
|
|
KEYWORD
|
|
Ecklonia cava extract, osteoclast, bone resorption, MAP kinases, heme oxygenase-1
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|